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mouse anti dsn1 (Novus Biologicals)

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Novus Biologicals mouse anti dsn1
Mouse Anti Dsn1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+dsn1/DSN1+Antibody+(2A7)/pmc11544903-86-84-86
Average 92 stars, based on 1 article reviews

mouse anti dsn1 - by Bioz Stars, 2026-09

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Article Title: Old Passengers as New Drivers: Chromosomal Passenger Proteins Engage in Translesion Synthesis
Article Snippet: The antibodies used were as follows: human CREST serum (Antibodies Incorporated, Davis, CA, USA, 15-234; 1:400), rabbit anti-53BP1 (Novus Biologicals, Centennial, CO, USA, NB100-304; 1:2000), rabbit anti-Aurora B (Sigma Aldrich/Merck KGaA, Darmstadt, Germany, A5102; 1:2000), mouse anti-pATM (Santa Cruz Biotechnology, Heidelberg, Germany, sc-47739; 1:500), mouse anti-Borealin (MBL/Biozol, Eching, Germany, M147-3; 1:200), rabbit anti-CENP-C (Biozol, Eching, Germany, A06766-1; 1:100), rabbit anti-CENP-F (Novus Biologicals, Centennial, CO, USA, NB500-101; 1:750), mouse anti-cyclin A2 (Santa Cruz Biotechnology, Heidelberg, Germany, sc-271682; 1:100), rabbit anti-pDNA-PKcs (Abcam, Cambridge, UK, ab18192; 1:1000), mouse anti-DSN1 (Novus Biologicals, Centennial, CO, USA, 2A7; 1:800), mouse anti-HA (BioLegend, San Diego, CA, USA, 901501; 1:1000), mouse anti-Hec1 (GeneTex, Irvine, CA, USA, GTX70268; 1:1000), rabbit anti-INCENP (NEB/Cell Signaling, New England BioLabs, Frankfurt am Main, Germany, 2807; 1:400), mouse anti-myc (NEB/Cell Signaling, New England BioLabs, Frankfurt am Main, Germany, 2276; 1:1500), mouse anti-NSL1 (Origene, OTI4D3; 1:800), mouse anti-PCNA (NEB/Cell Signaling, New England BioLabs, Frankfurt am Main, Germany, 2586; 1:3200), rabbit anti-Survivin (Novus Biologicals, Centennial, CO, USA, NB200-201; 1:300), and mouse anti-γH2AX (BioLegend, San Diego, CA, USA, 613402; 1:10,000).

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Anaphase error correction and micronuclei prevention requires stable kinetochore-microtubule interactions (A) HeLa cells stably expressing H2B-GFP and mRFP-α-tubulin treated with scramble small interfering RNA (siRNA) (control) or siNdc80, siKNL1, siMis12, siCENP-E, siCENP-T, siCENP-H, and siAurora B. Time 00:00, anaphase onset. White arrowheads indicate lagging chromosomes and arrows show MN. Scale bar represents 5 μm. (B) Western blot analysis showing efficient depletion of Ndc80 complex members, Dsn1, CENP-E, Aurora B, and Aurora A after RNAi. GAPDH or α-tubulin were used as loading controls. (C) Cell fate of mitotic HeLa cells under the specified conditions. Control n = 897, siNdc80 n = 202, siNuf2 n = 204, siSpc25 n = 222, siSpc24 n = 182, siKNL1 n = 194, siMis12 n = 200, siDsn1 n = 459, siNsl1 n = 200, siZw10 n = 187, siCENP-E n = 159, siCENP-T n = 150, siCENP-H n = 125, siAurora B n = 287, and siAurora A n = 229 cells. (D) Quantitative analysis of the time between nuclear envelope breakdown (NEB) and anaphase onset (error bars indicate mean ± SD; analyzed using an unpaired t test). (E) Frequency of anaphase cells with lagging chromosomes for all conditions (data were pooled from 2 independent experiments, analyzed using the Fisherś exact two-tailed test). (F) Frequency of dividing cells that form MN (left column) and of dividing cells with lagging chromosomes that form MN (right column). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.

Journal: Cell Reports

Article Title: An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors

doi: 10.1016/j.celrep.2021.109783

Figure Lengend Snippet: Anaphase error correction and micronuclei prevention requires stable kinetochore-microtubule interactions (A) HeLa cells stably expressing H2B-GFP and mRFP-α-tubulin treated with scramble small interfering RNA (siRNA) (control) or siNdc80, siKNL1, siMis12, siCENP-E, siCENP-T, siCENP-H, and siAurora B. Time 00:00, anaphase onset. White arrowheads indicate lagging chromosomes and arrows show MN. Scale bar represents 5 μm. (B) Western blot analysis showing efficient depletion of Ndc80 complex members, Dsn1, CENP-E, Aurora B, and Aurora A after RNAi. GAPDH or α-tubulin were used as loading controls. (C) Cell fate of mitotic HeLa cells under the specified conditions. Control n = 897, siNdc80 n = 202, siNuf2 n = 204, siSpc25 n = 222, siSpc24 n = 182, siKNL1 n = 194, siMis12 n = 200, siDsn1 n = 459, siNsl1 n = 200, siZw10 n = 187, siCENP-E n = 159, siCENP-T n = 150, siCENP-H n = 125, siAurora B n = 287, and siAurora A n = 229 cells. (D) Quantitative analysis of the time between nuclear envelope breakdown (NEB) and anaphase onset (error bars indicate mean ± SD; analyzed using an unpaired t test). (E) Frequency of anaphase cells with lagging chromosomes for all conditions (data were pooled from 2 independent experiments, analyzed using the Fisherś exact two-tailed test). (F) Frequency of dividing cells that form MN (left column) and of dividing cells with lagging chromosomes that form MN (right column). ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.

Article Snippet: The primary antibodies used were: anti-rabbit Mklp2/Kif20A (Bethyl Laboratories; 1:5000); anti-rabbit phospho Aurora A/B/C (T288/232/398; Cell Signaling; 1:2000); anti-rabbit Phospho-Histone H3 Ser10 (Monoclonal; D2C8; Cell Signaling Technology; 1:5000); anti-mouse Hec1 (9GA) (Monoclonal; Abcam; 1:1000), anti-mouse Dsn1 (a gift from A. Musacchio, 1:500), anti-mouse CENP-E (Monoclonal; Santa Cruz Biotechnology; 1:500), anti-mouse Aurora B (Monoclonal, BD Bioscience, 1:1000), anti-mouse GAPDH (Monoclonal; Proteintech; 1:40000), anti-mouse α-tubulin (Monoclonal; Sigma, 1:5000).

Techniques: Stable Transfection, Expressing, Small Interfering RNA, Control, Western Blot, Two Tailed Test

Journal: Cell Reports

Article Title: An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors

doi: 10.1016/j.celrep.2021.109783

Figure Lengend Snippet:

Techniques: Phospho-proteomics, Recombinant, Sequencing, Control, Software